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克隆号为H09的IDH1（isocitrate dehydrogenase 1, 异柠檬酸盐脱氢酶1）鼠单克隆抗体为Dianova公司近期推出的产品之一，此抗体可特异性的检测脑组织肿瘤中IDH1基因的R132H点突变。IDH1基因的第132密码子的杂合突变是II 和III级神经胶质瘤（根据WHO的肿瘤学分类）的频发事件。在大约70％的星形胶质细胞瘤和少突神经胶质细胞瘤可发现IDH1基因的R132H点突变。

IDH1基因R132H突变在部分脑肿瘤中的频发现象使克隆号为H09的IDH1抗体可高灵敏性和高特异性的用于此类肿瘤的鉴别诊断中：如从原发性胶质母细胞瘤中区分出间变性星形细胞瘤、从毛细胞型星形细胞瘤或室管膜（细胞）瘤中区分出弥漫性星形细胞瘤（WHO分类II级），也可以用于具有IDH1突变的肿瘤细胞浸润前沿的与反应性神经胶质增多症的区分。因此，由于其高灵敏性和高特异性，此抗体在脑部肿瘤的分类分级中以及在检测单一或少量浸润肿瘤细胞时是非常有用的。

DIA-H09-L – Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) – unconj. – 8 ml

Art.-Nr.                         DIA-H09-L

Specificity                      IDH1 R132H

Species Reactivity               Human

Host Species                     Mouse

Isotype                          IgG2a

Clone                             H09

Clonality (Mono-/Polyclonal)      monoclonal

Application                       Immunohistochemistry (IHC)

                                 Immunohistochemistry (Paraffin-embedded Sections)

Conjugation                       unconjugated

Dilution                          pre-diluted – ready-to-use

Format                             Ready-to-use

                                   prediluted antibody
                                   2% BSA
                                   0.05% NaN3

Product line / Topic                neurobiology

                                    tumor marker / biology
                                    immunohistochemistry

Intended Use                        for Research Use Only

Manufacturer / Brand                dianova

Reactivity

Antibody clone H09 reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximay 70% of astrocytomas and oligodendroglial tumors. The high frequency and distribution of the IDH1 R132H mutation among specific brain tumor entities allow the highly sensitive and specific discrimination of various tumors by immunohistochemistry, such as anaplastic astrocytoma from primary glioblastoma or diffuse astrocytoma WHO grade II from pilocytic astrocytoma or ependymoma. Noteworthy is the discrimination of the infiltrating edge of tumors with IDH1 mutation from reactive gliosis. This antibody is highly useful for tumor classification and in detecting single infiltrating tumor cells.

Immunohistochemistry of human IDH1 R132H in formalin-fixed paraffin-embedded brain tissue sections

A: Strong reaction of IDH1 mutation specific antibody clone H09 in tumor center of anaplastic oligoastrocytoma.
B: Infiltration zone of anaplastic astrocytoma with specific labelling of infiltrating glioma cells by antibody clone H09.
C: Identification of single tumor cells in white matter distant from tumor center with IDH1 mutation specific antibody clone H09.
D: Cortex infiltrated by oligodendroglioma with specific labelling of tumor cells by antibody clone H09.
E: Double staining of GFAP (glial fibrillary acidic protein, red) and clone H09 (brown) of oligodendroglioma infiltration zone demonstrating specific labelling of tumor cells but not of GFAP positive reactive astrocytes.
F: Strong reaction of IDH1 mutation specific antibody clone H09 with IDH1 R132H mutated diffuse astrocytoma (left) but not with wild type tumor (right).

A

B

C

D

E

F

(pictures courtesy of Prof. Dr. med. Andreas von Deimling, Department of Neuropathology, University Heidelberg / Clinical Co-operation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany)

References

1. Capper D et al. Monoclonal antibody specific for IDH1 R132H mutation. Acta Neuropathol. 118(5): 599-601, 2009
2. Capper D et al. Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol. 20(1): 245-254, 2010
3. Preusser M et al. IDH testing in diagnostic neuropathology: review and practical guideline article invited by the Euro-CNS research committee. Clinical Neuropathology, 30(5):217-230, 2011
4. Van den Bent MJ et al. Interlaboratory comparison of IDH mutation detection. J Neurooncol 112:173–178, 2013
5. Schumacher T et al. A vaccine targeting mutant IDH1 induces antitumour immunity. Nature 2014, DOI:10.1038/nature13387
6. David NL et al. The 2016 World Health Organization Classification of Tumors of the Central Nervous System: a summary. Acta Neuropathol., 131:803-820, 2016

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